During studies on the relationship between PyK and the Crabtree effect, the following facts were revealed: ( 1) The oxidation of succinate, pyruvate and NADH was inhibited by the substrate of PyK, PEP, while glycolysis of PEP was inhibited by succinate and NADH. 在研究PyK和克奈特瑞效应关系时发现:1.PyK的底物PEP可抑制线粒体中琥珀酸、丙酮酸和NADH的氧化,而琥珀酸和NADH又可抑制PEP的酵解;